Clonagem de Bambus Nativos da Amazônia Sul-Ocidental

Abstract

In vitro propagation is a biotechnological tool used in plant tissue culture for the mass propagation of species of forestry, agronomic, medicinal and ornamental interest, applied in studies of plant diversity and conservation. The process for obtaining a cloned seedling takes place under laboratory conditions, which reproduces an aseptic environment, free of contamination and with controlled environmental variables. The importance of this technique is related to the availability of healthy seedlings, that is, free of fungi, bacteria or nematodes. Obtaining it generally takes less time than the conventional process, and with the same characteristics as the matrix plant selected for cloning. For the production of seedlings in the laboratory, it is necessary to plan phases, such as: a) choice the plant that will be used as matrix; b) preparation of the culture medium; c) handling and inoculation of plant material (explant); d) incubation in a culture room for multiplication and rooting; e) pre acclimatization in laboratory or acclimatization in nursery. If necessary, seedlings from the laboratory must pass through the greenhouse to adapt to environmental conditions.